Genotyping-by-Sequencing for the Study of Genetic Diversity in Puccinia triticina.
Identifieur interne : 000071 ( Main/Exploration ); précédent : 000070; suivant : 000072Genotyping-by-Sequencing for the Study of Genetic Diversity in Puccinia triticina.
Auteurs : Meriem Aoun ; James A. Kolmer [États-Unis] ; Matthew Breiland ; Jonathan Richards ; Robert S. Brueggeman ; Les J. Szabo [États-Unis] ; Maricelis Acevedo [États-Unis]Source :
- Plant disease [ 0191-2917 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- Wicri :
English descriptors
- KwdEn :
- MESH :
- geographic : Mexico, Morocco.
- Genotype, Phylogeny, Plant Diseases.
Abstract
Leaf rust, caused by Puccinia triticina Erikss., is globally the most widespread rust of wheat. Populations of P. triticina are highly diverse for virulence, with many different races found annually. The genetic diversity of P. triticina populations has been previously assessed using different types of DNA markers. Genotyping technologies that provide a higher density of markers distributed across the genome will be more powerful for analysis of genetic and phylogenetic relationships in P. triticina populations. In this study, we utilized restriction-associated DNA (RAD) genotyping-by-sequencing (GBS) adapted for the Ion Torrent sequencing platform for the study of population diversity in P. triticina. A collection of 102 isolates, collected mainly from tetraploid and hexaploid wheat, was used. The virulence phenotypes of the isolates were determined on 20 lines of Thatcher wheat near isogenic for leaf rust resistance genes. Seven races were found among 57 isolates collected from tetraploid wheat, and 21 races were observed among 40 hexaploid wheat type isolates. This is the first study to report durum wheat virulent races to Lr3bg in Tunisia, Lr14a in Morocco, and Lr3bg and Lr28 in Mexico. Ethiopian isolates with high virulence to durum wheat but avirulent on Thatcher (hexaploid wheat) were tested for virulence on a set of durum (tetraploid) differentials. A subset of 30 isolates representing most of the virulence phenotypes in the 102 isolates were genotyped using RAD-GBS. Phylogenetic analysis of 30 isolates using 2,125 single nucleotide polymorphism (SNP) markers showed nine distinct clusters. There was a general correlation between virulence phenotypes and SNP genotypes. The high bootstrap values between clusters of isolates in the phylogenetic tree indicated that RAD-GBS can be used as a new genotyping tool that is fast, simple, high throughput, cost effective, and provides a sufficient number of markers for the study of genetic diversity in P. triticina.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
DOI: 10.1094/PDIS-09-19-1890-RE
PubMed: 31910116
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genotyping-by-Sequencing for the Study of Genetic Diversity in <i>Puccinia triticina</i>.</title><author><name sortKey="Aoun, Meriem" sort="Aoun, Meriem" uniqKey="Aoun M" first="Meriem" last="Aoun">Meriem Aoun</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Kolmer, James A" sort="Kolmer, James A" uniqKey="Kolmer J" first="James A" last="Kolmer">James A. Kolmer</name><affiliation wicri:level="2"><nlm:affiliation>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Minnesota, St. Paul</wicri:cityArea></affiliation></author><author><name sortKey="Breiland, Matthew" sort="Breiland, Matthew" uniqKey="Breiland M" first="Matthew" last="Breiland">Matthew Breiland</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Richards, Jonathan" sort="Richards, Jonathan" uniqKey="Richards J" first="Jonathan" last="Richards">Jonathan Richards</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Brueggeman, Robert S" sort="Brueggeman, Robert S" uniqKey="Brueggeman R" first="Robert S" last="Brueggeman">Robert S. Brueggeman</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Szabo, Les J" sort="Szabo, Les J" uniqKey="Szabo L" first="Les J" last="Szabo">Les J. Szabo</name><affiliation wicri:level="2"><nlm:affiliation>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Minnesota, St. Paul</wicri:cityArea></affiliation></author><author><name sortKey="Acevedo, Maricelis" sort="Acevedo, Maricelis" uniqKey="Acevedo M" first="Maricelis" last="Acevedo">Maricelis Acevedo</name><affiliation wicri:level="2"><nlm:affiliation>International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31910116</idno><idno type="pmid">31910116</idno><idno type="doi">10.1094/PDIS-09-19-1890-RE</idno><idno type="wicri:Area/Main/Corpus">000088</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000088</idno><idno type="wicri:Area/Main/Curation">000088</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000088</idno><idno type="wicri:Area/Main/Exploration">000088</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genotyping-by-Sequencing for the Study of Genetic Diversity in <i>Puccinia triticina</i>.</title><author><name sortKey="Aoun, Meriem" sort="Aoun, Meriem" uniqKey="Aoun M" first="Meriem" last="Aoun">Meriem Aoun</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Kolmer, James A" sort="Kolmer, James A" uniqKey="Kolmer J" first="James A" last="Kolmer">James A. Kolmer</name><affiliation wicri:level="2"><nlm:affiliation>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Minnesota, St. Paul</wicri:cityArea></affiliation></author><author><name sortKey="Breiland, Matthew" sort="Breiland, Matthew" uniqKey="Breiland M" first="Matthew" last="Breiland">Matthew Breiland</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Richards, Jonathan" sort="Richards, Jonathan" uniqKey="Richards J" first="Jonathan" last="Richards">Jonathan Richards</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Brueggeman, Robert S" sort="Brueggeman, Robert S" uniqKey="Brueggeman R" first="Robert S" last="Brueggeman">Robert S. Brueggeman</name><affiliation><nlm:affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</nlm:affiliation><wicri:noCountry code="subField">58108</wicri:noCountry></affiliation></author><author><name sortKey="Szabo, Les J" sort="Szabo, Les J" uniqKey="Szabo L" first="Les J" last="Szabo">Les J. Szabo</name><affiliation wicri:level="2"><nlm:affiliation>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Minnesota, St. Paul</wicri:cityArea></affiliation></author><author><name sortKey="Acevedo, Maricelis" sort="Acevedo, Maricelis" uniqKey="Acevedo M" first="Maricelis" last="Acevedo">Maricelis Acevedo</name><affiliation wicri:level="2"><nlm:affiliation>International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca</wicri:cityArea></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Genotype (MeSH)</term><term>Mexico (MeSH)</term><term>Morocco (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Diseases (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Génotype (MeSH)</term><term>Maladies des plantes (MeSH)</term><term>Maroc (MeSH)</term><term>Mexique (MeSH)</term><term>Phylogenèse (MeSH)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Mexico</term><term>Morocco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genotype</term><term>Phylogeny</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Génotype</term><term>Maladies des plantes</term><term>Maroc</term><term>Mexique</term><term>Phylogenèse</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Mexique</term><term>Maroc</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Leaf rust, caused by <i>Puccinia triticina</i> Erikss., is globally the most widespread rust of wheat. Populations of <i>P. triticina</i> are highly diverse for virulence, with many different races found annually. The genetic diversity of <i>P. triticina</i> populations has been previously assessed using different types of DNA markers. Genotyping technologies that provide a higher density of markers distributed across the genome will be more powerful for analysis of genetic and phylogenetic relationships in <i>P. triticina</i> populations. In this study, we utilized restriction-associated DNA (RAD) genotyping-by-sequencing (GBS) adapted for the Ion Torrent sequencing platform for the study of population diversity in <i>P. triticina</i>. A collection of 102 isolates, collected mainly from tetraploid and hexaploid wheat, was used. The virulence phenotypes of the isolates were determined on 20 lines of Thatcher wheat near isogenic for leaf rust resistance genes. Seven races were found among 57 isolates collected from tetraploid wheat, and 21 races were observed among 40 hexaploid wheat type isolates. This is the first study to report durum wheat virulent races to <i>Lr3bg</i> in Tunisia, <i>Lr14a</i> in Morocco, and <i>Lr3bg</i> and <i>Lr28</i> in Mexico. Ethiopian isolates with high virulence to durum wheat but avirulent on Thatcher (hexaploid wheat) were tested for virulence on a set of durum (tetraploid) differentials. A subset of 30 isolates representing most of the virulence phenotypes in the 102 isolates were genotyped using RAD-GBS. Phylogenetic analysis of 30 isolates using 2,125 single nucleotide polymorphism (SNP) markers showed nine distinct clusters. There was a general correlation between virulence phenotypes and SNP genotypes. The high bootstrap values between clusters of isolates in the phylogenetic tree indicated that RAD-GBS can be used as a new genotyping tool that is fast, simple, high throughput, cost effective, and provides a sufficient number of markers for the study of genetic diversity in <i>P. triticina</i>.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">31910116</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>05</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>104</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Genotyping-by-Sequencing for the Study of Genetic Diversity in <i>Puccinia triticina</i>.</ArticleTitle><Pagination><MedlinePgn>752-760</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-09-19-1890-RE</ELocationID><Abstract><AbstractText>Leaf rust, caused by <i>Puccinia triticina</i> Erikss., is globally the most widespread rust of wheat. Populations of <i>P. triticina</i> are highly diverse for virulence, with many different races found annually. The genetic diversity of <i>P. triticina</i> populations has been previously assessed using different types of DNA markers. Genotyping technologies that provide a higher density of markers distributed across the genome will be more powerful for analysis of genetic and phylogenetic relationships in <i>P. triticina</i> populations. In this study, we utilized restriction-associated DNA (RAD) genotyping-by-sequencing (GBS) adapted for the Ion Torrent sequencing platform for the study of population diversity in <i>P. triticina</i>. A collection of 102 isolates, collected mainly from tetraploid and hexaploid wheat, was used. The virulence phenotypes of the isolates were determined on 20 lines of Thatcher wheat near isogenic for leaf rust resistance genes. Seven races were found among 57 isolates collected from tetraploid wheat, and 21 races were observed among 40 hexaploid wheat type isolates. This is the first study to report durum wheat virulent races to <i>Lr3bg</i> in Tunisia, <i>Lr14a</i> in Morocco, and <i>Lr3bg</i> and <i>Lr28</i> in Mexico. Ethiopian isolates with high virulence to durum wheat but avirulent on Thatcher (hexaploid wheat) were tested for virulence on a set of durum (tetraploid) differentials. A subset of 30 isolates representing most of the virulence phenotypes in the 102 isolates were genotyped using RAD-GBS. Phylogenetic analysis of 30 isolates using 2,125 single nucleotide polymorphism (SNP) markers showed nine distinct clusters. There was a general correlation between virulence phenotypes and SNP genotypes. The high bootstrap values between clusters of isolates in the phylogenetic tree indicated that RAD-GBS can be used as a new genotyping tool that is fast, simple, high throughput, cost effective, and provides a sufficient number of markers for the study of genetic diversity in <i>P. triticina</i>.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aoun</LastName><ForeName>Meriem</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kolmer</LastName><ForeName>James A</ForeName><Initials>JA</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1790-202X</Identifier><AffiliationInfo><Affiliation>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul, MN 55108.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Breiland</LastName><ForeName>Matthew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Richards</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brueggeman</LastName><ForeName>Robert S</ForeName><Initials>RS</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Szabo</LastName><ForeName>Les J</ForeName><Initials>LJ</Initials><AffiliationInfo><Affiliation>United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, St. Paul, MN 55108.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Acevedo</LastName><ForeName>Maricelis</ForeName><Initials>M</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-4257-9375</Identifier><AffiliationInfo><Affiliation>International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008800" MajorTopicYN="N" Type="Geographic">Mexico</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009018" MajorTopicYN="N" Type="Geographic">Morocco</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="Y">Plant Diseases</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">cereals and grains</Keyword><Keyword MajorTopicYN="N">field crops</Keyword><Keyword MajorTopicYN="N">fungi</Keyword><Keyword MajorTopicYN="N">pathogen diversity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31910116</ArticleId><ArticleId IdType="doi">10.1094/PDIS-09-19-1890-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Minnesota</li><li>État de New York</li></region></list><tree><noCountry><name sortKey="Aoun, Meriem" sort="Aoun, Meriem" uniqKey="Aoun M" first="Meriem" last="Aoun">Meriem Aoun</name><name sortKey="Breiland, Matthew" sort="Breiland, Matthew" uniqKey="Breiland M" first="Matthew" last="Breiland">Matthew Breiland</name><name sortKey="Brueggeman, Robert S" sort="Brueggeman, Robert S" uniqKey="Brueggeman R" first="Robert S" last="Brueggeman">Robert S. Brueggeman</name><name sortKey="Richards, Jonathan" sort="Richards, Jonathan" uniqKey="Richards J" first="Jonathan" last="Richards">Jonathan Richards</name></noCountry><country name="États-Unis"><region name="Minnesota"><name sortKey="Kolmer, James A" sort="Kolmer, James A" uniqKey="Kolmer J" first="James A" last="Kolmer">James A. Kolmer</name></region><name sortKey="Acevedo, Maricelis" sort="Acevedo, Maricelis" uniqKey="Acevedo M" first="Maricelis" last="Acevedo">Maricelis Acevedo</name><name sortKey="Kolmer, James A" sort="Kolmer, James A" uniqKey="Kolmer J" first="James A" last="Kolmer">James A. Kolmer</name><name sortKey="Szabo, Les J" sort="Szabo, Les J" uniqKey="Szabo L" first="Les J" last="Szabo">Les J. Szabo</name><name sortKey="Szabo, Les J" sort="Szabo, Les J" uniqKey="Szabo L" first="Les J" last="Szabo">Les J. Szabo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RustFungiV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000071 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000071 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RustFungiV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31910116
|texte= Genotyping-by-Sequencing for the Study of Genetic Diversity in Puccinia triticina.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31910116" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RustFungiV1
| This area was generated with Dilib version V0.6.38. |  |